Insulin in Cats: Which One?

P. Jane Armstrong, DVM, MS, MBA, Dipl. ACVIM

University of Minnesota

 

Diabetes mellitus is the second most common endocrine disorder in cats, (behind hyperthyroidism), with an estimated incidence of 1 in 200 cats. Diabetes occurs in a wide age range of cats, but most cats are over 8 years of age when diagnosed. Neutered males are affected most frequently and obesity is a risk factor. Genetics may play a role in some breeds (e.g., Burmese cats in Australia).

   

Insulin Preparations 

There are three general types of insulin:  Human recombinant insulin  (e.g,. Humulin, Novolin), animal-source insulin (e.g., pork regular insulin and 90% beef-10% pork PZI insulin), and synthetic insulin (e.g., glargine). Insulins are categorized based on their onset and duration of action in human patients as:

1.      very fast-acting (e.g., Humalog and Novolog)

2.      fast-acting (e.g., Humulin R or Novolin R)

3.      intermediate-acting (e.g., the lente series of insulins, as well as PZI and NPH and pork Lente [Vetsulin])

4.      long-acting (e.g., Humulin U and Insulin detemir [Letemir])

5.      ultra long-acting (Lantus [glargine])

6.      mixtures (e.g., Humulin 70/30 which is 70% NPH and 30% regular insulin)

 

Production of lente and ultralente insulins has recently ceased due to waning use in human diabetics. Pork-origin NPH insulin has also recently left the market. Insulin glargine (Lantus, Aventis Pharmaceuticals) is a relatively new, synthetic, “peakless” insulin that differs from human insulin in that one amino acid is replaced by glycine and two arginines. Insulin glargine is currently recommended in human diabetics as a basal insulin to be administered once daily, at bedtime, with the goal of inhibiting hepatic glucose production. This ultra long-acting insulin analog forms microprecipitates at the site of injection from which small amounts of insulin are slowly released. This effect is a property of the low pH of the injected insulin.

 

Most insulin preparations are available as 100 units/ml (U-100); some are available as 40 units/ml (U-40). Insulin syringes are calibrated in units and must match insulin concentration (e.g., use U-100 syringes for U-100 insulin). Low-dose U-100 syringes are available (0.3 ml, 0.5 ml) and can be very useful for cats. Many insulin preparations can be diluted to facilitate more reliable administration of small volumes of insulin, but it is important to use a manufacturer-approved diluent and follow the manufacturer’s guidelines for duration of storage of diluted insulin (generally one month maximum). Insulin glargine cannot be mixed with other insulins or diluted, as altering the pH alters the absorption characteristics.

 

Unlike other insulins, glargine does not need to be kept refrigerated. Glargine has a shelf life of 4 weeks when kept at room temperature. When refrigerated, opened vials can be used for at least 6 months. If an insulin pen is used, the manufacturer recommends that the pen be kept at room temperature, as temperature changes associated with refrigeration alter the volume of insulin delivered by the pen.

Insulin Selection

Until recently, most endocrinologists recommended starting diabetic cats on either recombinant lente insulin or PZI of beef/pork origin. Insulin glargine has recently replaced lente and PZI as the insulin of choice in cats. A prospective study was done comparing results with lente, PZI, and glargine in diabetic cats. Twenty-four consecutive newly diabetic cats were enrolled in the study and randomly assigned to receive therapy with glargine, PZI or lente insulin. All were fed a low-carbohydrate diet. Diabetic remission was achieved in 8/8 cats receiving glargine, 3/8 receiving PZI, and 2/8 receiving lente. Diabetic remission occurred within 4 months in all cases, with the median time to remission being 3 weeks. The results from this study suggest that glargine is a better choice than PZI in diabetic cats because glycemic control was better and diabetic remission (reversion to a non-diabetic state) rates were higher.

 

Studies in healthy cats suggest that insulin glargine and PZI  have similar pharmacokinetic properties. Studies of glargine in diabetic cats have yet to be reported, but this insulin appears to have a duration of effect ranging from 10 to 16 hours in most diabetic cats, with significant suppression of blood glucose for up to 24 hours. Although many cats may potentially be able to be maintained on once-daily dosing with glargine, the limited data available suggest that optimal glycemic control with minimal risk of clinical hypoglycemia occurs with twice-daily dosing.

Beginning Therapy with Insulin Glargine

Insulin glargine should be dosed according to initial blood glucose at diagnosis and ideal body weight. If the blood glucose is equal to or greater than 360 mg/dl, start with 0.5 U/kg twice daily subcutaneously, and if the blood glucose is less than 360 mg/dl, start with 0.25 U/kg twice daily. A 12-hour serial blood glucose curve should be obtained with samples taken every 4 hours. Because of the long duration of action and likely overlap on insulin action, the dose should not be increased for the first week. The dose should be decreased, however, if blood glucose concentration drops below the reference range. Note that many cats have little decrease in blood glucose over the first 3 days of treatment with glargine, even if diabetic remission is achieved within 4 weeks.

Newly-diagnosed non-ketotic diabetic cats are typically hospitalized for 3 days to begin therapy with insulin glargine or the owner may elect to monitor blood glucose at home for the first 3 days to check the initial response. If the cat was admitted with ketoacidosis, stabilize the cat with regular insulin according to described protocols, and use the above guidelines once the patient is judged ready to receive longer-acting subcutaneous insulin injections. It is important to search diligently for concurrent disease in all newly-diagnosed diabetic cats as the advanced age of most diabetics predisposes to other diseases and because some diseases predispose to diabetes. 

Monitoring

Response to treatment can be evaluated in a variety of ways, including clinical signs, changes in body weight and water intake, and blood glucose results. Repeat 12-hour blood glucose curves are obtained at weeks 1,2, and 4 and then as required. Adjustments in insulin therapy are made gradually until an effective insulin treatment protocol is identified. Dosage changes are made based on a number of blood glucose parameters and, ideally, changes should be based on more than one parameter. The preinsulin blood glucose has been found to be important when using glargine or PZI because of a persisting effect from the previous injection and the possibility of diabetic remission. The preinsulin blood glucose is used to decide whether to continue to administer insulin.  Nadir (lowest) blood glucose can be used to adjust the dose, as with other insulin types. Measurement of water intake at home is simpler, less expensive and less stressful than measuring serum fructosamine concentration, and correlates better with daily blood glucose concentration. Glargine offers new hope for diabetic cats that are fractious, as dosage changes can be made relatively safely based on water intake, urine glucose concentration, and clinical assessment.

Table 1. Parameters for changing insulin dosage when using glargine or PZI in diabetic cats (adapted from Rand, JS and Marshall RD. Vet Clinics Small Anim 2005;35, 211-224.)

Parameter used for dosage adjustment

Change in dose

Preinsulin BG >360 mg/dl and/or nadir >180 mg/dl

Increase by 0.5-1.0 U

Preinsulin BG 270 to <360 mg/dl and/or nadir 90-180 mg/dl

No dose change

Preinsulin BG 190-270 mg/dl and/or nadir 54-72 mg/dl

Use nadir, water drunk, urine glucose, and next preinsulin BG to determine whether to decrease or maintain dose

Preinsulin BG <180 mg/dl and/or nadir <54 mg/dl

Reduce dose by 0.5-1.0 U or if total dose is 0.5-1 U, stop insulin and check for diabetic remission

If clinical signs of hypoglycemia are observed

Reduce dose by 50%

If water intake <20 ml/kg on canned food or <70 ml/kg on dry food

No dose change

If water intake >40 ml/kg on canned food or >100 ml/kg on dry food

Increase by 0.5-1.0 U

If urine glucose is >3+

Increase by 0.5-1.0 U

If urine glucose is negative

Reduce dose by 1 U and check for diabetic remission if on a low insulin dose

Determining if Diabetic Remission has Occurred

It is suggested that insulin therapy be continued for a minimum of 2 weeks to allow pancreatic beta cells to recover fully from glucose toxicity. After a minimum of 2 weeks of insulin therapy, if the preinsulin blood glucose is <200 mg/dl and the insulin dose is only 0.5-1 U per cat once or twice a day, insulin should be withheld and a 12-hour blood glucose curve performed. If the blood glucose concentration exceeds 200 mg/dl at the next scheduled dosing time, administer 1 U twice daily; if the blood glucose is 200 mg/dl or less, continue to withhold insulin and discharge with a follow up visit in one week.

Dietary Recommendations

Correction of obesity and minimizing the impact of the diet on postprandial blood glucose concentration are important dietary considerations in diabetic cats. Obesity is common in the age population of cats that are at risk for diabetes; over 40% of cats aged 9-13 in the US are overweight or obese. This is also the peak age of occurrence of diabetes mellitus. Obesity causes reversible insulin resistance which resolves as obesity is corrected. Although there are several diets specifically formulated for weight reduction in cats, diets that minimize intestinal carbohydrate absorption should be fed to diabetic cats.

The central theme in studies evaluating the impact of diet on glycemic control in diabetic cats has been restriction of carbohydrate absorption by the gastrointestinal tract, either by inhibiting starch digestion (acarbose), inhibiting intestinal glucose absorption (fiber), or decreasing carbohydrate ingestion (low carbohydrate-containing diets). Intuitively, the most effective means to minimize gastrointestinal absorption of carbohydrates in the diabetic cat is to feed diets that contain minimal amounts of carbohydrate and this approach has proven very effective. Current recommendations are to feed a low carbohydrate such as Nestle Purina DM or Hill’s m/d. The beneficial role, if any, of the high protein or high fat content of these carbohydrate-restricted diets on control of glycemia is not known at this time. These dietary recommendations are intended to augment and not replace insulin therapy. If an alternative to insulin therapy is sought, consideration should be given to an oral hypoglycemic gent and a low carbohydrate diet. This may be a useful approach while the owner of a newly diagnosed diabetic adjusts to the reality of giving insulin injections. Websites that may be helpful to owners of diabetic cats include <www.veterinarypartner.com> and <www.felinediabetes.com>.

Selected References

Marshall RD and Rand JS. Treatment with glargine results in higher remission rates than lente or protamine zinc insulins in newly diagnosed diabetic cats [abstract]. J Vet Intern Med 2005; 19:425.

Marshall RD and Rand JS. Comparison of the pharmacokinetics and pharmacodynamics of glargine, protamine zinc and porcine lente insulins in normal cats [abstract]. J Vet Intern Med 2002; 16(3), 358.

Mazzaferro EM, Greco DS, Turner AS, et al. Treatment of feline diabetes mellitus using an alpha-glucosidase inhibitor and a low-carbohydrate diet. J Feline Med Surg 2003; 5(3), 183-9.

Rand, JS and Marshall RD. Diabetes mellitus in cats. Vet Clinics Small Anim 2005;35, 211-224.